Unusual kinetic behaviour of plasmin (fibrinolysin) digestion of fibrin and N-benzyloxycarbonyltyrosine nitrophenyl ester.

Tissue necrosis may be detected by the appearance of intracellular enzymes in extracellular spaces (Kibe & Nilsson, 1967) and this principle has been applied to ischaemic and hypoxic cardiac necrosis in man and animals (Roe & Starmer, 1975; Shell et al., 1973). Beating myocardial cells in culture represent an extremely reproducible preparation for studies of the deleterious effect of hypoxia on cardiac tissue. The aim of the study reported here was to examine the metabolic basis of the exacerbation of damage caused by increased medium CaZf concentration to heart cells subjected to anoxia.